Associate Principal Scientist Veranova Westford, Massachusetts
The increasing complexity of therapeutic modalities such as peptides and antibody-drug conjugates (ADCs) requires advanced analytical tools that deliver accurate, high-throughput data. Multi-Angle Light Scattering (MALS), when coupled with Size Exclusion Chromatography (SEC), offers a powerful technique for determining absolute molecular weight, aggregation, and sample heterogeneity without reliance on molecular standards. This presentation explores how SEC-MALS enhances the sensitivity, specificity, and data quality of bioanalytical workflows by providing orthogonal confirmation of size and purity profiles. Particular attention is given to the method’s advantages in characterizing conjugated and non-conjugated biotherapeutics, ensuring consistency during development and manufacturing. Attendees will gain a clear understanding of how SEC-MALS fits within modern analytical strategies for novel modalities, supporting regulatory compliance and product development. This session is designed for scientists seeking practical, efficient solutions for chemical characterization in early- and late-stage development of complex biologics.
Learning Objectives:
Upon completion, participants will be able to describe the principles and applications of SEC-MALS for the analytical characterization of peptides and ADCs.
Upon completion, participants will be able to explain how SEC-MALS measures absolute molecular weight and size without the need for calibration standards.
Upon completion, participants will be able to differentiate the analytical needs of peptides versus ADCs and how SEC-MALS addresses them.
Upon completion, participants will be able to interpret SEC-MALS results to assess sample purity, aggregation, and heterogeneity.
Upon completion, participants will be able to identify best practices and common pitfalls when applying SEC-MALS in bioanalysis.
